Home > IVD Products > ImmunoAssays > Cardiovascular & Salt Balance > Renin > Renin Direct, ELISA, 96 tests

Renin Direct, ELISA, 96 tests

The DIAsource Renin-ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplates. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human Renin – MAb 2 – HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB) is added and incubated. The reaction is stopped with the addition of Stop solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the human Renin concentration. A calibration curve is plotted and Renin concentration in samples is determined by interpolation from the calibration curve.

Catalog # KAP1531
Format ELISA
Label HRP
Size 96 tests
Sample Type EDTA Plasma
Sample Volume 200 µL
Controls 2 levels
Range 4-270 pg/mL
Sensitivity 0,8 pg/mL
Incubation 1h/1h/30min at RT with shaking 400 rpm
Shelf Life (weeks) 48

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAP1531.pdf
Download "Material Safety Data Sheet" MSKAP1531.pdf
Download catalogue "ELISA" CATE.pdf
Bridging Science And Diagnostics
Choose my country

DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

Please select your country to see the most accurate content available in your area.