After extraction of the plasma samples, angiotensin II is assayed by a competitive radioimmunoassay. This radioimmunoassay is using a rabbit anti-angiotensin II antiserum and a radio-iodinated angiotensin II tracer. Bound and free phases are separated by a second antibody bound to solid phase particles, followed by a centrifugation step. The radioactivity in the bound fractions is measured and a typical calibration curve can be generated.
Catalog # | RB320RUO |
Format | RIA |
Label | I125 |
Size | 100 tests |
Sample Type | Plasma |
Sample Volume | 1 mL |
Controls | 2 levels |
Range | 4,7-150 pmol/L |
Sensitivity | 2 pmol/L |
Incubation | 6h + Overnight + 30min + 15min at 2-8°C |
Shelf Life (weeks) | 12 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.