CanAg PSA - ELISA

The CanAg PSA EIA kit is intended for the quantitative determination of Total PSA (Prostate Specific Antigen) in human serum.

Dertermination of PSA is now widely used for detection and management of patients with prostatic cancer and conidered as the superior serological marker for cancer of the prostate.

The CanAg PSA EIA is a non-competitive solid-phase immunoassay based on the direct sandwich technique.

Calibrators, Controls and patient samples are incubated together with biotinylated Anti-PSA monoclonal antibody and raIFO/rt peroxidase (HRP) labeled Anti-PSA monoclonal antibody, in Steptavidin-coated microtiter strips.

After washing, the buffered Substrate/Chromogen reagent (hydrogen peroxide and 3, 3', 5, 5' tetra methyl benzidine) is added to each well and the enzymatic reaction can begin.

During the enzymatic reaction, a blue color develops, if the antigen is present. The intensity of the color is proportional to the amount of PSA present in the samples. Color intensity is determined by a microtiter plate spectrophotometer at 620 nm (or optionally at 405 nm after addition of Stop Solution).

Calibration curves are constructed for each test by plotting absorbance values against the concentration of each Calibrator. The PSA concentrations of the patient samples are then read from the calibration curve.