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25OH Vitamin D Total ELISA 90', 96 tests

The DIAsource 25OH Vitamin D Total ELISA 90’ is a competitive ELISA assay, based on patented monoclonal antibodies, with a novel pre-treatment step performed inside the ELISA microtiter plate.
It is the fastest 25OH Vitamin D ELISA assay on the market !

The assay is an upgrade of the well-established 25OH Vitamin D Total ELISA (launched in 2012) and is based on the same key technologies:
- Pre-treatment inside the ELISA microplate
- Patented Vitamin D Monoclonal Antibody
- Proprietary Vitamin D conjugate

The assay features both a short incubation time (90’) and a short hands-on time (45’ for a total plate).

The assay shows very competitive sensitivity, precision and performance characteristics to all other immunoassays in the market but uses a novel and extremely easy pre-treatment step performed directly in the microtiter plate. It keeps the same advantages as the 25OH Vitamin D Total ELISA:
- Pre-treatment in the ELISA microplate
- 100% automatable
- Breakable wells format
- Calibration to Thienpont’s Reference Measurement Procedure
- 24 months shelf life

And offers other advantages :
- Lower sample volume (25 µL)
- Validated for the use of serum and plasma
- Available as a 1 plate (96 Tests) and 2 plates (192 Tests) version: (Same box = gain in transport and storage; Cheaper price per test; Open stability of 8 weeks at 4°C)

The novel approach of the DIAsource 25OH Vitamin D Total ELISA 90’ makes it extremely easy to program on any open ELISA platform without the need for a manual sample pre-treatment outside the ELISA automate. The assay does not require a manual transfer from extracted sample in a tube to the well in the microtiter plate.

Catalog # KAP1971-F1
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, Plasma
Sample Volume 25 µL
Controls 2 levels
Range 4,9-121 ng/mL
Sensitivity 3,04 ng/mL
Pretreatment Direct pretreatment
Incubation 60min./15 min./15 min at RT with shaking 400 rpm
Shelf Life (weeks) 144

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAP1971-F1.pdf
Download "Material Safety Data Sheet" MSKAP1971-F1.pdf
Download catalogue "Vitamin D" CATV.pdf
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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