The DIAsource 25OH Vitamin D Total ELISA 90’ is a competitive ELISA assay, based on patented monoclonal antibodies, with a novel pre-treatment step performed inside the ELISA microtiter plate.
It is the fastest 25OH Vitamin D ELISA assay on the market !
The assay is an upgrade of the well-established 25OH Vitamin D Total ELISA (launched in 2012) and is based on the same key technologies:
- Pre-treatment inside the ELISA microplate
- Patented Vitamin D Monoclonal Antibody
- Proprietary Vitamin D conjugate
The assay features both a short incubation time (90’) and a short hands-on time (45’ for a total plate).
The assay shows very competitive sensitivity, precision and performance characteristics to all other immunoassays in the market but uses a novel and extremely easy pre-treatment step performed directly in the microtiter plate. It keeps the same advantages as the 25OH Vitamin D Total ELISA:
- Pre-treatment in the ELISA microplate
- 100% automatable
- Breakable wells format
- Calibration to Thienpont’s Reference Measurement Procedure
- 24 months shelf life
And offers other advantages :
- Lower sample volume (25 µL)
- Validated for the use of serum and plasma
- Available as a 1 plate (96 Tests) and 2 plates (192 Tests) version: (Same box = gain in transport and storage; Cheaper price per test; Open stability of 8 weeks at 4°C)
The novel approach of the DIAsource 25OH Vitamin D Total ELISA 90’ makes it extremely easy to program on any open ELISA platform without the need for a manual sample pre-treatment outside the ELISA automate. The assay does not require a manual transfer from extracted sample in a tube to the well in the microtiter plate.
| Catalog # | KAP1971-F1 |
| Format | ELISA |
| Label | HRP |
| Size | 96 tests |
| Sample Type | Serum, Plasma |
| Sample Volume | 25 µL |
| Controls | 2 levels |
| Range | 0-135 ng/mL |
| Sensitivity | 4,12 ng/mL |
| Pretreatment | Direct pretreatment |
| Incubation | 60min./15 min./15 min at RT with shaking 400 rpm |
| Shelf Life (weeks) | 104 |
| Label |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KAP1971-F1.pdf |  |
| Download "Material Safety Data Sheet" | MSKAP1971-F1.pdf | |
| Download brochure "25OH Vitamin D Total ELISA 90' Assay | CATV.pdf | |
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