The DIAsource 1,25(OH)2 Vitamin D RIA is a competitive RIA assay, based on the Gold Standard extraction methodology.
The assay shows equal or superior sensitivity, precision and cross-reactivity profile to all other RIA assays in the market.
Only samples and controls, not the calibrators, are extracted with a mix of solvents and applied on cartridges to separate 1,25(OH)2 Vitamin-D from other Vitamin-D metabolites. After elution of samples and controls, the calibrators, samples and controls are incubated in coated tubes. A fixed amount of 125I labelled 1,25(OH)2 Vitamin D competes with the 1,25(OH)2 Vitamin D to be measured present in the sample or in the calibrator for a fixed amount of antibody sites immobilized on the wall of a polystyrene tube. After an overnight incubation at room temperature, an aspiration step terminates the competition reaction. The tubes are then washed with washing solution and aspirated. A calibration curve is plotted and the 1,25(OH)2 Vitamin D concentrations of the samples are determined by dose interpolation from the calibration curve.
A RUO version is available for US customers. Contact our customer services for more information.
Download our Technical Review on Performance of the DIAsource 1,25(OH)2 Vitamin D RIA assay in DEQAS and Instand Quality Control Programs.
Download our Technical Review on Technical Review 2015-05 - Performance of the DIAsource 1,25(OH)2 Vitamin D RIA assay in DEQAS Quality Control Program.
Download our Technical Review on Technical Review 2015-06 - Correlation of the DIAsource 1,25(OH)2 Vitamin D RIA assay with LC-MS.
Download our Technical Review on Technical Review 2015-07 - Evaluation of the DIAsource 25OH Vitamin D Total RIA assay according to the Vitamin D Standardization Program (VDSP).
| Catalog # | KIP1929 |
| Format | RIA CT |
| Label | I125 |
| Size | 48 tests |
| Sample Type | Serum, Plasma |
| Sample Volume | 500 µL |
| Controls | 2 levels |
| Range | 4.7-411 pg/mL |
| Sensitivity | 1.6 pg/mL |
| Incubation | Overnight |
| Shelf Life (weeks) | 10 |
Iodination Schedule 2015 :
Due to local registration requirement, this product can not be sold in Canada, United States – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: RA@diasource.be.
| Download "Instructions for Use" | KIP1929.pdf |  |
| Download "Material Safety Data Sheet" | MSKIP1929.pdf | |
| Download brochure "1,25(OH)2 Vitamin D RIA Assay" | 125ohr.pdf | |
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