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Adrenaline, ELISA, 96 tests

Adrenaline (Epinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte concentrations compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Catalog # KAPL10-0100
Format ELISA
Label HRP
Size 96 tests
Sample Type EDTA Plasma, Urine
Sample Volume 300/10 µL
Controls 2 levels
Range 1-256 ng/mL
Sensitivity 330 pg/mL for urine - 11 pg/mL for plasma
Incubation 4.5 hours at RT with shaking at 400-500 rpm
Shelf Life (weeks) 60

Due to local registration requirement, this product can not be sold in Canada, United States – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: RA@diasource.be.

Documents - Download
Download "Instructions for Use" KAPL10-0100.pdf
Download "Material Safety Data Sheet" MSKAPL10-0100.pdf
Download brochure "Biogenic Amines I" CATE.pdf
Coordinates

DIAsource ImmunoAssays SA
BE 0457.934.723
Rue du Bosquet, 2 | 1348 Louvain-la-Neuve - Belgium
Tel +32 10 84 99 11 | Fax +32 10 84 99 90

CLOSING DAYS 2019 : 1/01-22/04-01&30/05-10/06-21/07-15/08-01&11/11-25/12

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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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