5-HIAA, ELISA, 96 tests

5-HIAA is quantitatively derivatized by methylation. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Catalog # KAPL10-1900
Format ELISA
Label HRP
Size 96 tests
Sample Type Urine
Sample Volume 50 µL
Controls 2 levels
Range 0,17-50 mg/L
Sensitivity 0.17 mg/L
Incubation 3h
Shelf Life (weeks) 60

Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KAPL10-1900.pdf
Download "Material Safety Data Sheet" MSKAPL10-1900.pdf
Download catalogue "ELISA" CATE.pdf
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