
Adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are extracted by using a cis-diol- specific affinity gel, acylated and then converted enzymatically. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.
Catalog # | KIPL1600 |
Format | RIA |
Label | I125 |
Size | 3 x 96 tests |
Sample Type | EDTA Plasma, Urine |
Sample Volume | 300 µL (Plasma) /10 µL (Urine) |
Controls | 2 levels |
Range | Urine Adr: 0,39-200 ng/ml Urine Nor: 1,1-1000 ng/ml Urine Dop: 3 - 2000 ng/ml Plasma Adr: 19 -6667 pg/ml Plasma Nor: 42 - 33333 pg/ml Plasma Dop: 29 - 26667 pg/ml |
Sensitivity | Urine Adr: 0,2 ng/ml Urine Nor: 1,1 ng/ml Urine Dop: 3 ng/ml Plasma Adr: 4,1 pg/ml Plasma Nor: 42 pg/ml Plasma Dop: 29 pg/ml |
Incubation | 4h or Overnight |
Shelf Life (weeks) | 14 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.