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3-CAT, RIA, 3 x 96 tests

Adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are extracted by using a cis-diol- specific affinity gel, acylated and then converted enzymatically. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.

Catalog # KIPL1600
Format RIA
Label I125
Size 3 x 96 tests
Sample Type EDTA Plasma, Urine
Sample Volume 300 µL (Plasma) /10 µL (Urine)
Controls 2 levels
Range Urine Adr: 0,39-200 ng/ml Urine Nor: 1,1-1000 ng/ml Urine Dop: 3 - 2000 ng/ml Plasma Adr: 19 -6667 pg/ml Plasma Nor: 42 - 33333 pg/ml Plasma Dop: 29 - 26667 pg/ml
Sensitivity Urine Adr: 0,2 ng/ml Urine Nor: 1,1 ng/ml Urine Dop: 3 ng/ml Plasma Adr: 4,1 pg/ml Plasma Nor: 42 pg/ml Plasma Dop: 29 pg/ml
Incubation 4h or Overnight
Shelf Life (weeks) 14

For registration requirements in countries outside of European market, please contact regulatory.affairs@diasource.be.

Documents - Download
Download "Instructions for Use" KIPL1600.pdf
Download "Material Safety Data Sheet" MSKIPL1600.pdf
Download catalogue "RIA" CATRv2201.pdf
Download RIA Availability Calendar
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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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