Adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are extracted by using a cis-diol- specific affinity gel, acylated and then converted enzymatically. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.
| Catalog # | KIPL1600 |
| Format | RIA |
| Label | I125 |
| Size | 3 x 96 tests |
| Sample Type | EDTA Plasma, Urine |
| Sample Volume | 300 µL (Plasma) /10 µL (Urine) |
| Controls | 2 levels |
| Range | Urine Adr: 0,39-200 ng/ml Urine Nor: 1,1-1000 ng/ml Urine Dop: 3 - 2000 ng/ml Plasma Adr: 19 -6667 pg/ml Plasma Nor: 42 - 33333 pg/ml Plasma Dop: 29 - 26667 pg/ml |
| Sensitivity | Urine Adr: 0,2 ng/ml Urine Nor: 1,1 ng/ml Urine Dop: 3 ng/ml Plasma Adr: 4,1 pg/ml Plasma Nor: 42 pg/ml Plasma Dop: 29 pg/ml |
| Incubation | 4h or Overnight |
| Shelf Life (weeks) | 14 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KIPL1600.pdf |  |
| Download "Material Safety Data Sheet" | MSKIPL1600.pdf | |
| Download catalogue "RIA" | ||
| Download RIA Availability Calendar |
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