First, the plasma proteins are removed by precipitation. Then the metanephrine (metadrenaline) and normetanephrine (normetadrenaline) are quantitatively acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.
| Catalog # | KIPL1400 |
| Format | RIA |
| Label | I125 |
| Size | 2 x 100 tests |
| Sample Type | EDTA Plasma |
| Sample Volume | 200 µL |
| Controls | 2 levels |
| Range | M: 6,4-3600 pg/ml N: 24,1-7200 pg/ml |
| Sensitivity | M: 5,8 pg/ml N: 21,4 pg/ml |
| Incubation | Overnight at 2-8°C |
| Shelf Life (weeks) | 14 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.
| Download "Instructions for Use" | KIPL1400.pdf |  |
| Download "Material Safety Data Sheet" | MSKIPL1400.pdf | |
| Download catalogue "RIA" | ||
| Download RIA Availability Calendar |
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