First, the plasma proteins are removed by precipitation. Then the metanephrine (metadrenaline) and normetanephrine (normetadrenaline) are quantitatively acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.
Catalog # | KIPL1400 |
Format | RIA |
Label | I125 |
Size | 2 x 100 tests |
Sample Type | EDTA Plasma |
Sample Volume | 200 µL |
Controls | 2 levels |
Range | M: 6,4-3600 pg/ml N: 24,1-7200 pg/ml |
Sensitivity | M: 5,8 pg/ml N: 21,4 pg/ml |
Incubation | Overnight at 2-8°C |
Shelf Life (weeks) | 14 |
Due to local registration requirement, this product can not be sold in Canada, United States, Australia – without prior registration.
For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: regulatory.affairs@diasource.be.