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Gliadin IgG, ELISA, 96 tests

The DIAWell Gliadin IgG kit is a solid phase enzyme immunoassay using 96 coated breakaway microwells and a peroxidase-TMB detection system. The microwells are coated with highly specific antigen. In the test procedure, serum samples are diluted 1/51 and incubated in the microwells. Human antibodies, if present, bind to the specific antigen. Unbound or excess antibodies are removed by washing and HRP-conjugated rabbit antibodies against human IgG are added to the microwells. The enzyme conjugate binds to the antigen-antibody complexes. After a second washing step to remove excess conjugate, the TMB/substrate solution is added. The enzyme activity, if present, generates a colorimetric (blue) reaction. Diluted acid is added to stop the reaction. Consequently the colour turns from blue to yellow and may be measured at 450 nm using a conventional microplate reader. The absorbance (Optical Density) is directly proportional to the concentration of IgG antibodies bound to the antigen on the microwells surface

Catalog # KAPDTGLG02
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum
Sample Volume 10 µL
Controls 2 levels
Range 25-400 U/mL
Sensitivity 70.60%
Incubation 30min/30min/10min RT
Shelf Life (weeks) 52

Due to local registration requirement, this product can not be sold in Italy, Canada, United States, Australia – without prior registration.

For Japan and Brazil, specific registration requirements are necessary. For more information, please contact: RA@diasource.be.

Documents - Download
Download "Instructions for Use" KAPDTGLG02.pdf
Download "Material Safety Data Sheet" MSKAPDTGLA02.pdf
Download brochure Marketing CATE.pdf

DIAsource ImmunoAssays SA
BE 0457.934.723
Rue du Bosquet, 2 | 1348 Louvain-la-Neuve - Belgium
Tel +32 10 84 99 11 | Fax +32 10 84 99 90

CLOSING DAYS 2019 : 1/01-22/04-01&30/05-10/06-21/07-15/08-01&11/11-25/12

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DIAsource ImmunoAssays (formerly BioSource), experienced diagnostic manufacturer of RIA-ELISA-LIA immunoassays, validation on automates, large scale production of Antibodies-Point of Care Tests.

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