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HBV, Anti-HBs Ag, ELISA, 96 tests

Anti-HBs Elisa is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immuno-sorbent assay ) based on the "sandwich principle". The solid phase of the microtiter plate is made of polystyrene wells coated with HBsAg (subtype Ad and Ay), and the liquid phase of peroxidase conjugated HBsAg (subtype Ad and Ay). When a serum or plasma specimen containing Anti-HBs is added to the HBsAg-coated wells together with the peroxidase conjugated HBsAg and incubated, (antigen)-(Anti-HBs)-(antigen • peroxidase) complexes will form on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. A color develops in proportion to the amount of Anti-HBs bound to HBsAg. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450 nm with a selected reference wavelength within 620 to 690 nm.

Catalog # KAPG4SBE3
Format ELISA
Label HRP
Size 96 tests
Sample Type Serum, Plasma
Sample Volume 50 µL
Controls 2 levels
Sensitivity 3.6 mIU/mL
Calibration WHO Standard W1042

Download "Instructions for Use"

Download "Material Safety Data Sheet"

Download brochure "Hepatitis"

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